Investigation of antioxidant and antimicrobial activities of walnut (Juglans regia L.) kernel septum

Objectives: Walnut (Juglans regia L.) kernel septum (or septa) (WKS), a traditional nutraceutical material in China, has not been explored detail. In this study, antimicrobial activity, total phenolic content (TPC) and antioxidant-oxidant status of WKS was investigated case it may be clinically important the management various complications. Methods: The extracted with ethanol Soxhlet device. TPC analysed by using Folin-Ciocalteu’s method. Antioxidant activity obtained Rel Assay Diagnostics kits. evaluated against two Gram-positive (Staphylococcus aureus, Bacillus subtilis), one Gram-negative bacteria (Escherichia coli) fungus (Candida albicans) strains agar diffusion Results: found to 119.42 ± 2.39 mg GAE/gDW. It determined that antioxidant (TAS), oxidant (TOS) oxidative stress index (OSI) values were 7.542 0.389 mmol/L, 3.718 0.287 µmol/L 0.049 0.001, respectively. selectively inhibited growth fungus, while S. aureus most susceptible 16 mm inhibition zone. resistant extract. Conclusions: As far as we know, paper is first work demonstrates method described above, moreover there are no scientific reports which have examined such multidisciplinary experimental design. This study strongly supports reported use WKS. Results indicated can used pharmacological natural agent due its high activities.